Facilities

The Regenerative Biomaterials and Therapeutics Group is located at the Pittsburgh Technology Center and operates a biomaterials and cell biology research lab as well as administering a number of shared-use instruments for the Biomedical Engineering Department and the Carnegie Institute of Technology.

Major Equipment

  1. Asylum Research MFP-3D-BIO Atomic Force Microscope
  2. Zeiss LSM 700 Laser Scanning Confocal Microscope
  3. Nikon AZ-C2 Laser Scanning Confocal Microscope
  4. Nikon SMZ1500 Epifluorescent Stereomicroscope
  5. Leica TP5 Multiphoton Microscope
  6. Nikon Ti-E Deconvolution Microscope
  7. Advanced Image Processing Workstation with Imaris and AutoQuant Deconvolution Software
  8. BD FACSJazz Fluorescently Activated Cell Sorter

Additional Resources

  1. Cell Culture Facilities
  2. Polymer Processing/Fabrication
  3. Rapid Prototyping and 3D Printing

Atomic Force Microscope


Asylum Research MFP-3d-BIO Atomic Force Microscope with Integrated Nikon Ti Inverted Fluorescent Microscope

The AFM is integrated with a Nikon Ti inverted epifluorescent microscope for coupled optical and AFM imaging of biological samples.  The Nikon Ti is equipped with 10x, 40x and 60x objectives, filter cubes for DAPI, FITC and TRITC and a Hamamatsu ORCA-Flash 2.8 Scientific CMOS camera.

This equipment is maintained by the Regenerative Biomaterials Group, but is a shared resource and is part of the Center for the Mechanics and Engineering of Cellular Systems.  Interested CMU students and faculty can gain access to this system after training.

New users must follow the training protocol.

 

 

Confocal Microscope Imaging Systems


Zeiss LSM 700 Laser Scanning Confocal Microscope

Fully motorized inverted microscope with full incubation and 4 laser lines.

 

 

 

 

 

 

 

 


Nikon AZ100 C2 Laser Scanning Confocal Microscope

Multizoom 8x macroscope with 1x and 5x objectives coupled to a C2 laser scanning confocal microscope (4 laser lines) for imaging embryos, tissue engineered constructs and other millimeter to microscale structures.

This equipment is a shared resource, interested CMU students and faculty should contact Prof. Feinberg for access.

 

 

 

 

 

Additional Optical Imaging Systems


Nikon TS100F Inverted Epifluorescence Microscope

Standard inverted epifluorescence microscope equipped with 5x, 10x, 20x and 40x objectives and DAPI, FITC and TRITC filter cubes.  Digital capture using a Photometrics CoolSnap ES or Cascade 1K CCD camera.

 

 

 

 

 

 

 


Nikon SMZ 1000 Stereomicroscope

 

 

 

 

 

 

 

 

 

Cell Culture Facilities

 

 

 

 

 

 

 

 

 

 

Polymer Processing/Fabrication